PCR amplification of markers was carried out in a PTC-200 Peltier Thermal Cycler (MJ Research, Inc.) in a total volume of 20µL with the following genotyping PCR reagents: 2µL of DNA at 20 ng/µL, 2µL of 10×buffer containing 25 mM MgCl2,1µL of
2.5 mM dNTPs, 1 unit of Taq Polymerase (Intron Biotechnology,Korea), and 1µL each of forward and reverse primers (10µM).